Hotspot

psbA-trnH-GUG

LSC · 5991-6293 · 303 bp

Back to Anemonastrum

Pi 0.0923

MarkerSeek score 84.00

Primer available yes

Label psbA-trnH-GUG

Feature Metadata

label_name: psbA-trnH-GUG
feature_type: igs
region: LSC
length_bp: 303
variable_sites: 45
pi: 0.0923
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.90
markerseek_score: 84.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbA-trnH-GUG_p1	TCTAGCGGCTGTTGAAGTTC	CGAGATATAGTGGGCGAACG	50.0	55.0	57.9	58.0	411-429 bp	1.0	91.1
psbA-trnH-GUG_p2	TTCCCTCTAGATCTAGCGGC	CGAGATATAGTGGGCGAACG	55.0	55.0	58.1	58.0	422-440 bp	1.0	91.0
psbA-trnH-GUG_p3	GGCTGTTGAAGTTCCATCCA	CGAGATATAGTGGGCGAACG	50.0	55.0	58.1	58.0	405-423 bp	1.0	91.0
psbA-trnH-GUG_p4	CCTCTAGATCTAGCGGCTGT	CGAGATATAGTGGGCGAACG	55.0	55.0	58.1	58.0	419-437 bp	1.0	91.0
psbA-trnH-GUG_p5	GCGAACCTTGGTATGGAAGT	CGAGATATAGTGGGCGAACG	50.0	55.0	57.9	58.0	467-485 bp	1.0	90.4