Hotspot

rps16

LSC · 4864-5989 · 1126 bp

Back to Androsace

Pi 0.0445

MarkerSeek score 81.30

Primer available yes

Label rps16

Feature Metadata

label_name: rps16
feature_type: gene
region: LSC
length_bp: 1126
variable_sites: 222
pi: 0.0445
unique_haplotype_count: 30
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 81.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16_p1	ACCACGCAATCCAAACATTG	AGGGGTTGAAAGAATTGCGA	45.0	45.0	57.6	57.7	2380-2421 bp	0.1	42.8
rps16_p2	TACCGTTGAGTTAGCAACCC	AGGGGTTGAAAGAATTGCGA	50.0	45.0	57.5	57.7	2444-2485 bp	0.1	42.6
rps16_p3	CCACGCAATCCAAACATTGA	AGGGGTTGAAAGAATTGCGA	45.0	45.0	57.3	57.7	2379-2420 bp	0.1	40.5
rps16_p4	ACTCATGTTGGATTGGCACT	AGGGGTTGAAAGAATTGCGA	45.0	45.0	57.4	57.7	2066-2080 bp	0.1	39.9
rps16_p5	CGTTGAGTTAGCAACCCGAA	AGGGGTTGAAAGAATTGCGA	50.0	45.0	58.5	57.7	2481-2482 bp	0.066667	38.1