Hotspot

rpl32-trnL-UAG

SSC · 110256-110980 · 725 bp

Back to Androsace

Pi 0.0989

MarkerSeek score 71.80

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: SSC
length_bp: 725
variable_sites: 212
pi: 0.0989
unique_haplotype_count: 28
primer_available: yes
alignment_reliability: 0.41
markerseek_score: 71.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	TTCCACTGGGCAGTCAAAAA	TGCCCCTTTTTGACGAATCA	45.0	45.0	57.8	58.0	662-1108 bp	0.2	58.7
rpl32-trnL-UAG_p2	TTTCCACTGGGCAGTCAAAA	TGCCCCTTTTTGACGAATCA	45.0	45.0	57.8	58.0	663-1109 bp	0.2	58.7
rpl32-trnL-UAG_p3	TTCCACTGGGCAGTCAAAAA	AAGAACACTTGGCGCAGTTA	45.0	45.0	57.8	58.0	850-1296 bp	0.2	58.5
rpl32-trnL-UAG_p4	TTCCACTGGGCAGTCAAAAA	TCATGCCCCTTTTTGACGAA	45.0	45.0	57.8	58.0	1085-1111 bp	0.133333	50.0
rpl32-trnL-UAG_p5	TTTCCACTGGGCAGTCAAAA	TCATGCCCCTTTTTGACGAA	45.0	45.0	57.8	58.0	1086-1112 bp	0.133333	50.0