Hotspot

psbE-petL

LSC · 61652-62926 · 1275 bp

Back to Androsace

Pi 0.0534

MarkerSeek score 83.20

Primer available yes

Label psbE-petL

Feature Metadata

label_name: psbE-petL
feature_type: igs
region: LSC
length_bp: 1275
variable_sites: 317
pi: 0.0534
unique_haplotype_count: 30
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 83.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE-petL_p1	AAAGGATCAAAACGGCCAGT	AGCGCTCTTAGTTCAGTTCG	45.0	50.0	58.0	58.0	1546-2084 bp	0.966667	90.5
psbE-petL_p2	AAGGATCAAAACGGCCAGTT	AGCGCTCTTAGTTCAGTTCG	45.0	50.0	58.0	58.0	1545-2083 bp	0.966667	90.5
psbE-petL_p3	AAAGGATCAAAACGGCCAGT	TTGGCAATTGGCTGGTAAGT	45.0	45.0	58.0	58.0	1147-1687 bp	0.833333	85.2
psbE-petL_p4	AAGGATCAAAACGGCCAGTT	TTGGCAATTGGCTGGTAAGT	45.0	45.0	58.0	58.0	1146-1686 bp	0.833333	85.2
psbE-petL_p5	GTCCGTGAATAGCCAACCAT	TTGGCAATTGGCTGGTAAGT	50.0	45.0	58.0	58.0	1248-1788 bp	0.8	83.6