Hotspot

trnF-GAA-ndhJ

LSC · 47614-48271 · 658 bp

Back to Androsace

Pi 0.0492
MarkerSeek score 87.10
Primer available yes
Label trnF-GAA-ndhJ

Feature Metadata

label_name
trnF-GAA-ndhJ
feature_type
igs
region
LSC
length_bp
658
variable_sites
162
pi
0.0492
unique_haplotype_count
30
primer_available
yes
alignment_reliability
0.98
markerseek_score
87.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnF-GAA-ndhJ_p1 GGGATAGCTCAGCTGGTAGA AAGGATTACATTGCCCCCAA 55.0 45.0 58.0 57.1 621-795 bp 0.966667 83.1
trnF-GAA-ndhJ_p2 CCTCGTGTCACCAGTTCAAA AAGGATTACATTGCCCCCAA 50.0 45.0 58.1 57.1 585-759 bp 0.966667 82.7
trnF-GAA-ndhJ_p3 CTGGTAGAGCAGAGGACTGA AAGGATTACATTGCCCCCAA 55.0 45.0 57.9 57.1 609-783 bp 0.966667 82.1
trnF-GAA-ndhJ_p4 GGGATAGCTCAGCTGGTAGA AGGATTACATTGCCCCCAAT 55.0 45.0 58.0 56.8 620-794 bp 0.966667 81.3
trnF-GAA-ndhJ_p5 CCTCGTGTCACCAGTTCAAA AGGATTACATTGCCCCCAAT 50.0 45.0 58.1 56.8 584-758 bp 0.966667 80.9