Hotspot

psaA-pafI

LSC · 40904-41589 · 686 bp

Back to Androsace

Pi 0.0445

MarkerSeek score 82.10

Primer available yes

Label psaA-pafI

Feature Metadata

label_name: psaA-pafI
feature_type: igs
region: LSC
length_bp: 686
variable_sites: 181
pi: 0.0445
unique_haplotype_count: 29
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 82.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaA-pafI_p1	GCCCATTCCTCGAAAGAAGT	ATCACTAGACGTTTCGAATAA	50.0	33.3	57.9	52.5	770-804 bp	1.0	52.0
psaA-pafI_p2	TTTTACTTCTGGTTCCGGCG	ATCACTAGACGTTTCGAATAA	50.0	33.3	58.5	52.5	723-757 bp	1.0	52.0
psaA-pafI_p3	GGCCCATTCCTCGAAAGAAG	ATCACTAGACGTTTCGAATAA	55.0	33.3	58.6	52.5	771-805 bp	1.0	52.0
psaA-pafI_p4	TTTACTTCTGGTTCCGGCG	ATCACTAGACGTTTCGAATAA	52.6	33.3	57.8	52.5	722-756 bp	1.0	52.0
psaA-pafI_p5	CTGGCCCATTCCTCGAAAG	ATCACTAGACGTTTCGAATAA	57.9	33.3	58.2	52.5	773-807 bp	0.966667	50.7