Hotspot

trnT-GGU-psbD

LSC · 30761-32042 · 1282 bp

Back to Androsace

Pi 0.0446

MarkerSeek score 86.10

Primer available yes

Label trnT-GGU-psbD

Feature Metadata

label_name: trnT-GGU-psbD
feature_type: igs
region: LSC
length_bp: 1282
variable_sites: 296
pi: 0.0446
unique_haplotype_count: 30
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 86.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-GGU-psbD_p1	GCCCTTTTAACTCAGCGGTA	ACAAAAACGAAACGGTCCCT	50.0	45.0	57.9	58.2	1022-1463 bp	0.966667	87.9
trnT-GGU-psbD_p2	GCCCTTTTAACTCAGCGGTA	GGACCAGCCTACAAAAACGA	50.0	50.0	57.9	58.1	1032-1473 bp	0.933333	87.6
trnT-GGU-psbD_p3	GGCGTAAGTCATCGGTTCAA	GGACCAGCCTACAAAAACGA	50.0	50.0	58.3	58.1	995-1436 bp	0.966667	87.5
trnT-GGU-psbD_p4	GCCCTTTTAACTCAGCGGTA	AGAGCAATAAACCGGACCAG	50.0	50.0	57.9	57.6	1045-1486 bp	0.933333	85.3
trnT-GGU-psbD_p5	GCCCTTTTAACTCAGCGGTA	CAAAAACGAAACGGTCCCTC	50.0	50.0	57.9	57.6	1343-1462 bp	0.9	84.0