Hotspot

rps16-trnQ-UUG

LSC · 5990-6996 · 1007 bp

Back to Androsace

Pi 0.0603

MarkerSeek score 79.70

Primer available yes

Label rps16-trnQ-UUG

Feature Metadata

label_name: rps16-trnQ-UUG
feature_type: igs
region: LSC
length_bp: 1007
variable_sites: 261
pi: 0.0603
unique_haplotype_count: 30
primer_available: yes
alignment_reliability: 0.78
markerseek_score: 79.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16-trnQ-UUG_p1	CGTTGCTTTCTACCACATCG	GAGGTTCGAATCCTTCCGTC	50.0	55.0	57.2	58.1	759-1085 bp	0.966667	83.8
rps16-trnQ-UUG_p2	GCACGTTGCTTTCTACCACA	GAGGTTCGAATCCTTCCGTC	50.0	55.0	59.1	58.1	762-1088 bp	1.0	82.9
rps16-trnQ-UUG_p3	CGTTGCTTTCTACCACATCG	TTCGGAGGTTCGAATCCTTC	50.0	50.0	57.2	57.3	763-1089 bp	0.966667	79.0
rps16-trnQ-UUG_p4	CGTTGCTTTCTACCACATCG	TGATCTCGGTATTCGGAGGT	50.0	50.0	57.2	57.6	774-1087 bp	0.9	78.8
rps16-trnQ-UUG_p5	GCACGTTGCTTTCTACCACA	TGATCTCGGTATTCGGAGGT	50.0	50.0	59.1	57.6	777-1090 bp	0.9	76.7