Hotspot

ndhA

SSC · 112770-114880 · 2111 bp

Back to Andropogon

Pi 0.00603

MarkerSeek score 65.10

Primer available yes

Label ndhA

Feature Metadata

label_name: ndhA
feature_type: gene
region: SSC
length_bp: 2111
variable_sites: 109
pi: 0.00603
unique_haplotype_count: 35
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 65.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhA_p1	TGAACCCAGTTAGCATAGGGA	ATGGGGGAAGTTGATCGTTG	47.6	50.0	58.2	57.9	2240-2286 bp	1.0	74.5
ndhA_p2	TGAACCCAGTTAGCATAGGGA	GGGGGAAGTTGATCGTTGAA	47.6	50.0	58.2	57.8	2238-2284 bp	1.0	74.0
ndhA_p3	CCCAGTTAGCATAGGGAACA	ATGGGGGAAGTTGATCGTTG	50.0	50.0	56.6	57.9	2236-2282 bp	1.0	72.6
ndhA_p4	TGAACCCAGTTAGCATAGGG	ATGGGGGAAGTTGATCGTTG	50.0	50.0	56.6	57.9	2240-2286 bp	1.0	72.6
ndhA_p5	ACCCAGTTAGCATAGGGAAC	ATGGGGGAAGTTGATCGTTG	50.0	50.0	56.6	57.9	2237-2283 bp	1.0	72.6