Hotspot

ccsA-ndhD

SSC · 108385-108564 · 180 bp

Back to Andropogon

Pi 0.0388

MarkerSeek score 68.90

Primer available yes

Label ccsA-ndhD

Feature Metadata

label_name: ccsA-ndhD
feature_type: igs
region: SSC
length_bp: 180
variable_sites: 32
pi: 0.0388
unique_haplotype_count: 25
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 68.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA-ndhD_p1	GCACTTGTAGCTTCGATAGGA	TCCGTTGATAGGGTAGAGGT	47.6	50.0	57.6	56.9	338-363 bp	0.973684	67.0
ccsA-ndhD_p2	GCACTTGTAGCTTCGATAGGA	TCCGTTGATAGGGTAGAGGTT	47.6	47.6	57.6	57.6	338-363 bp	0.973684	65.0
ccsA-ndhD_p3	GCACTTGTAGCTTCGATAGG	TCCGTTGATAGGGTAGAGGT	50.0	50.0	56.1	56.9	338-363 bp	1.0	64.4
ccsA-ndhD_p4	GCACTTGTAGCTTCGATAGGA	ATCCGTTGATAGGGTAGAGGT	47.6	47.6	57.6	57.4	339-364 bp	0.973684	63.2
ccsA-ndhD_p5	GCACTTGTAGCTTCGATAGGAT	TCCGTTGATAGGGTAGAGGT	45.5	50.0	58.0	56.9	338-363 bp	0.973684	62.4