Hotspot

psaI-ycf4

LSC · 57560-57941 · 382 bp

Back to Andropogon

Pi 0.0115

MarkerSeek score 67.30

Primer available yes

Label psaI-ycf4

Feature Metadata

label_name: psaI-ycf4
feature_type: igs
region: LSC
length_bp: 382
variable_sites: 39
pi: 0.0115
unique_haplotype_count: 30
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 67.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaI-ycf4_p1	TTTCCGGCAATTGCAATGAC	TCCATATGTGTTCTGATCGCC	45.0	47.6	57.9	57.9	441-470 bp	1.0	81.4
psaI-ycf4_p2	TTTCCGGCAATTGCAATGAC	CCATATGTGTTCTGATCGCCA	45.0	47.6	57.9	58.2	440-469 bp	1.0	81.1
psaI-ycf4_p3	TTTCCGGCAATTGCAATGAC	CCCCAACCGCTAAGAATCC	45.0	57.9	57.9	57.9	531-560 bp	1.0	80.9
psaI-ycf4_p4	TTTCCGGCAATTGCAATGAC	TGTGTTCTGATCGCCAATTCA	45.0	42.9	57.9	58.2	435-464 bp	1.0	80.6
psaI-ycf4_p5	TTTCCGGCAATTGCAATGAC	CCCAACCGCTAAGAATCCTA	45.0	50.0	57.9	56.7	530-559 bp	1.0	79.3