Hotspot

trnF-GAA-ndhJ

LSC · 48151-48741 · 591 bp

Back to Andropogon

Pi 0.0189

MarkerSeek score 66.90

Primer available yes

Label trnF-GAA-ndhJ

Feature Metadata

label_name: trnF-GAA-ndhJ
feature_type: igs
region: LSC
length_bp: 591
variable_sites: 80
pi: 0.0189
unique_haplotype_count: 31
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 66.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnF-GAA-ndhJ_p1	CCTCGTGTCACCAGTTCAAA	TGAAATACAAGATGCTCATTGA	50.0	31.8	58.1	53.8	614-695 bp	1.0	52.0
trnF-GAA-ndhJ_p2	AATCCTCGTGTCACCAGTTC	TGAAATACAAGATGCTCATTGA	50.0	31.8	57.5	53.8	617-698 bp	1.0	52.0
trnF-GAA-ndhJ_p3	CCTCGTGTCACCAGTTCAAA	ATGAAATACAAGATGCTCATTGA	50.0	30.4	58.1	54.4	615-696 bp	1.0	52.0
trnF-GAA-ndhJ_p4	ATGCACAAGAAAGGGTCAGG	TGAAATACAAGATGCTCATTGA	50.0	31.8	58.1	53.8	667-748 bp	0.973684	50.9
trnF-GAA-ndhJ_p5	GCACAAGAAAGGGTCAGGAT	TGAAATACAAGATGCTCATTGA	50.0	31.8	57.8	53.8	665-746 bp	0.973684	50.9