Hotspot

trnC-GCA-rpoB

LSC · 18818-19580 · 763 bp

Back to Andropogon

Pi 0.0163

MarkerSeek score 72.00

Primer available yes

Label trnC-GCA-rpoB

Feature Metadata

label_name: trnC-GCA-rpoB
feature_type: igs
region: LSC
length_bp: 763
variable_sites: 77
pi: 0.0163
unique_haplotype_count: 35
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 72.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnC-GCA-rpoB_p1	AAAGGATTTGCAGTCCCCTG	TCTCAAGTTCTTCCGCCAAG	50.0	50.0	58.1	57.8	915-1415 bp	1.0	85.9
trnC-GCA-rpoB_p2	AAAGGATTTGCAGTCCCCTG	CTTCTCAAGTTCTTCCGCCA	50.0	50.0	58.1	57.8	917-1417 bp	1.0	85.8
trnC-GCA-rpoB_p3	AAAGGATTTGCAGTCCCCTG	ACTTCTCAAGTTCTTCCGCC	50.0	50.0	58.1	57.8	918-1418 bp	1.0	85.8
trnC-GCA-rpoB_p4	GATTTTTGATCAGGCGGCAC	CTTCTCAAGTTCTTCCGCCA	50.0	50.0	58.1	57.8	956-1456 bp	0.947368	82.8
trnC-GCA-rpoB_p5	GATTTTTGATCAGGCGGCAC	TCTCAAGTTCTTCCGCCAAG	50.0	50.0	58.1	57.8	954-1454 bp	0.947368	82.8