Hotspot

rpoA

LSC · 75701-76720 · 1020 bp

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Pi 0.00143

MarkerSeek score 30.80

Primer available yes

Label rpoA

Feature Metadata

label_name: rpoA
feature_type: gene
region: LSC
length_bp: 1020
variable_sites: 8
pi: 0.00143
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 30.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoA_p1	CGAGTGGTACTTCTTTCCCG	TTCGTACAGTGGGTTTGCAA	55.0	45.0	58.0	58.0	1723-1732 bp	1.0	81.4
rpoA_p2	CGAGTGGTACTTCTTTCCCG	GATGTAACACCTATGCCGCA	55.0	50.0	58.0	58.1	1610-1619 bp	1.0	81.3
rpoA_p3	CGAGTGGTACTTCTTTCCCG	AAAGTGCGTATTGGTTTGCG	55.0	45.0	58.0	58.0	1949-1958 bp	1.0	81.1
rpoA_p4	CGGGCTTATTGACAGTACCC	TTCGTACAGTGGGTTTGCAA	55.0	45.0	58.1	58.0	1637-1646 bp	1.0	81.1
rpoA_p5	CGGGCTTATTGACAGTACCC	AAAGTGCGTATTGGTTTGCG	55.0	45.0	58.1	58.0	1863-1872 bp	1.0	80.8