Hotspot

rpl16

LSC · 79046-80518 · 1473 bp

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Pi 0.00402

MarkerSeek score 49.60

Primer available yes

Label rpl16

Feature Metadata

label_name: rpl16
feature_type: gene
region: LSC
length_bp: 1473
variable_sites: 53
pi: 0.00402
unique_haplotype_count: 16
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 49.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl16_p1	CCAGAAGGAAAAACCAGGGG	AGAAGAAAGTATCCCTCTTTC	55.0	38.1	58.4	52.3	1577-1718 bp	1.0	43.8
rpl16_p2	CAGAAGGAAAAACCAGGGGT	AGAAGAAAGTATCCCTCTTTC	50.0	38.1	57.6	52.3	1576-1717 bp	1.0	43.8
rpl16_p3	CCAGAAGGAAAAACCAGGGG	AAGAAGAAAGTATCCCTCTTTC	55.0	36.4	58.4	53.2	1578-1719 bp	1.0	43.8
rpl16_p4	CAGAAGGAAAAACCAGGGGT	AAGAAGAAAGTATCCCTCTTTC	50.0	36.4	57.6	53.2	1577-1718 bp	1.0	43.8
rpl16_p5	CCAGAAGGAAAAACCAGGGG	AAAGAAGAAAGTATCCCTCTTTC	55.0	34.8	58.4	54.0	1579-1720 bp	1.0	43.8