Hotspot

rps15-trnN-GUU

IRa · 117699-119293 · 1595 bp

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Pi 0.00106

MarkerSeek score 53.60

Primer available yes

Label rps15-trnN-GUU

Feature Metadata

label_name: rps15-trnN-GUU
feature_type: igs
region: IRa
length_bp: 1595
variable_sites: 10
pi: 0.00106
unique_haplotype_count: 12
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 53.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps15-trnN-GUU_p1	AATGTGAAGCAAGTCTCCGT	ACGACCAGTCAGTTAACAGC	45.0	50.0	57.4	57.8	1340-1739 bp	1.0	78.9
rps15-trnN-GUU_p2	AATGTGAAGCAAGTCTCCGT	AGGATTCGAACCTACGACCA	45.0	50.0	57.4	58.2	1353-1752 bp	1.0	78.8
rps15-trnN-GUU_p3	AATGTGAAGCAAGTCTCCGT	GGATTCGAACCTACGACCAG	45.0	55.0	57.4	57.8	1352-1751 bp	1.0	78.4
rps15-trnN-GUU_p4	AATGTGAAGCAAGTCTCCGT	ACCACTGAGCTACTGAGGAA	45.0	50.0	57.4	57.7	1310-1709 bp	1.0	77.6
rps15-trnN-GUU_p5	AATGTGAAGCAAGTCTCCGT	CCCCAAGTAGGATTCGAACC	45.0	55.0	57.4	57.7	1361-1760 bp	1.0	77.4