Hotspot

trnN-GUU-rps15

IRb · 102698-104292 · 1595 bp

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Pi 0.00111
MarkerSeek score 54.50
Primer available yes
Label trnN-GUU-rps15

Feature Metadata

label_name
trnN-GUU-rps15
feature_type
igs
region
IRb
length_bp
1595
variable_sites
11
pi
0.00111
unique_haplotype_count
13
primer_available
yes
alignment_reliability
1.00
markerseek_score
54.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnN-GUU-rps15_p1 ACGACCAGTCAGTTAACAGC AATGTGAAGCAAGTCTCCGT 50.0 45.0 57.8 57.4 1340-1739 bp 1.0 78.9
trnN-GUU-rps15_p2 AGGATTCGAACCTACGACCA AATGTGAAGCAAGTCTCCGT 50.0 45.0 58.2 57.4 1353-1752 bp 1.0 78.8
trnN-GUU-rps15_p3 GGATTCGAACCTACGACCAG AATGTGAAGCAAGTCTCCGT 55.0 45.0 57.8 57.4 1352-1751 bp 1.0 78.5
trnN-GUU-rps15_p4 ACCACTGAGCTACTGAGGAA AATGTGAAGCAAGTCTCCGT 50.0 45.0 57.7 57.4 1310-1709 bp 1.0 77.6
trnN-GUU-rps15_p5 CCCCAAGTAGGATTCGAACC AATGTGAAGCAAGTCTCCGT 55.0 45.0 57.7 57.4 1361-1760 bp 1.0 77.5