Hotspot

petL-petG

LSC · 64980-65150 · 171 bp

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Pi 0.0284

MarkerSeek score 56.80

Primer available yes

Label petL-petG

Feature Metadata

label_name: petL-petG
feature_type: igs
region: LSC
length_bp: 171
variable_sites: 11
pi: 0.0284
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 56.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petL-petG_p1	TGGTTTTCTACTGGCTGCTT	ACGCAGTCACGAATAATCCC	45.0	50.0	57.3	58.1	320 bp	1.0	77.2
petL-petG_p2	TTGGTTTTCTACTGGCTGCT	ACGCAGTCACGAATAATCCC	45.0	50.0	57.3	58.1	321 bp	1.0	77.2
petL-petG_p3	TGGTTTTCTACTGGCTGCTT	CAGTCACGAATAATCCCGCT	45.0	50.0	57.3	57.8	317 bp	1.0	76.0
petL-petG_p4	TTGGTTTTCTACTGGCTGCT	CAGTCACGAATAATCCCGCT	45.0	50.0	57.3	57.8	318 bp	1.0	76.0
petL-petG_p5	TGGTTTTCTACTGGCTGCTT	CGCCTGTATTGCAAATACGC	45.0	50.0	57.3	58.3	336 bp	1.0	75.5