Hotspot

trnF-GAA-ndhJ

LSC · 49280-49870 · 591 bp

Back to Anatherum

Pi 0.00777

MarkerSeek score 56.30

Primer available yes

Label trnF-GAA-ndhJ

Feature Metadata

label_name: trnF-GAA-ndhJ
feature_type: igs
region: LSC
length_bp: 591
variable_sites: 20
pi: 0.00777
unique_haplotype_count: 11
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 56.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnF-GAA-ndhJ_p1	CCTCGTGTCACCAGTTCAAA	TGAAAGTTGGATAGGCTGGC	50.0	50.0	58.1	57.9	668-726 bp	1.0	84.9
trnF-GAA-ndhJ_p2	ATGCACAAGAAAGGGTCAGG	TGAAAGTTGGATAGGCTGGC	50.0	50.0	58.1	57.9	721-817 bp	1.0	84.4
trnF-GAA-ndhJ_p3	CCTCGTGTCACCAGTTCAAA	ATAGGCTGGCCCTTACGTAA	50.0	50.0	58.1	58.2	658-716 bp	1.0	84.3
trnF-GAA-ndhJ_p4	ATGCACAAGAAAGGGTCAGG	ATAGGCTGGCCCTTACGTAA	50.0	50.0	58.1	58.2	711-807 bp	1.0	83.8
trnF-GAA-ndhJ_p5	GCACAAGAAAGGGTCAGGAT	TGAAAGTTGGATAGGCTGGC	50.0	50.0	57.8	57.9	719-815 bp	1.0	83.5