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atpI-atpH

LSC · 32851-33685 · 835 bp

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Pi 0.00154

MarkerSeek score 51.50

Primer available yes

Label atpI-atpH

Feature Metadata

label_name: atpI-atpH
feature_type: igs
region: LSC
length_bp: 835
variable_sites: 9
pi: 0.00154
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 51.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpI-atpH_p1	TAGCCGCAGCCTATATAGGT	CAGTACCTTGACCAACTCCG	50.0	55.0	57.5	57.9	949-976 bp	1.0	77.9
atpI-atpH_p2	ATTTTTGCAACGTTAGCCGC	CAGTACCTTGACCAACTCCG	45.0	55.0	58.6	57.9	962-989 bp	1.0	77.4
atpI-atpH_p3	TAGCCGCAGCCTATATAGGT	AAGCAGCAGCAATTAGTGGA	50.0	45.0	57.5	57.8	883-910 bp	1.0	77.1
atpI-atpH_p4	TAGCCGCAGCCTATATAGGT	GCAGTACCTTGACCAACTCC	50.0	55.0	57.5	58.2	950-977 bp	1.0	77.0
atpI-atpH_p5	TAGCCGCAGCCTATATAGGT	GCCAATCCAGCAGCAATAAC	50.0	50.0	57.5	57.8	905-932 bp	1.0	76.9