Hotspot

rpl22-rps19

LSC · 84677-84778 · 102 bp

Back to Anaphalis

Pi 0.0202

MarkerSeek score 64.60

Primer available yes

Label rpl22-rps19

Feature Metadata

label_name: rpl22-rps19
feature_type: igs
region: LSC
length_bp: 102
variable_sites: 16
pi: 0.0202
unique_haplotype_count: 11
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 64.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl22-rps19_p1	TAACTCTTCGCGCTTTGTGA	TTGGGAGAATTCGCACCTAC	45.0	50.0	57.9	57.6	238-287 bp	1.0	85.4
rpl22-rps19_p2	TAACTCTTCGCGCTTTGTGA	TGGGAGAATTCGCACCTACT	45.0	50.0	57.9	58.4	237-286 bp	1.0	85.2
rpl22-rps19_p3	GAACGCCCACGAATTTGATC	TTGGGAGAATTCGCACCTAC	50.0	50.0	57.8	57.6	260-309 bp	1.0	84.7
rpl22-rps19_p4	GAACGCCCACGAATTTGATC	TGGGAGAATTCGCACCTACT	50.0	50.0	57.8	58.4	259-308 bp	1.0	84.5
rpl22-rps19_p5	TAACTCTTCGCGCTTTGTGA	AATTGGGAGAATTCGCACCT	45.0	45.0	57.9	57.5	240-289 bp	1.0	84.4