Hotspot

rps11-rpl36

LSC · 80231-80330 · 100 bp

Back to Anaphalis

Pi 0.00884

MarkerSeek score 57.20

Primer available yes

Label rps11-rpl36

Feature Metadata

label_name: rps11-rpl36
feature_type: igs
region: LSC
length_bp: 100
variable_sites: 14
pi: 0.00884
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 57.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps11-rpl36_p1	ACGTGAACCCTTTTTCGGTA	AAGAGCTTCCGTTCGTAAAA	45.0	40.0	57.4	55.3	190-239 bp	1.0	64.2
rps11-rpl36_p2	ACGTGAACCCTTTTTCGGTA	ATTTGTTCCAACCCAAGACA	45.0	40.0	57.4	55.3	123-172 bp	0.978723	63.1
rps11-rpl36_p3	ACGTGAACCCTTTTTCGGTA	TTTGTTCCAACCCAAGACAT	45.0	40.0	57.4	55.3	122-171 bp	0.978723	63.1
rps11-rpl36_p4	TCCACGTGAACCCTTTTTCG	TTTGTTCCAACCCAAGACAT	50.0	40.0	58.7	55.3	125-174 bp	0.978723	62.4
rps11-rpl36_p5	TCCACGTGAACCCTTTTTCG	ATTTGTTCCAACCCAAGACA	50.0	40.0	58.7	55.3	126-175 bp	0.978723	62.3