Hotspot

ndhC-trnV-UAC

LSC · 51158-52384 · 1227 bp

Back to Anaphalis

Pi 0.0119

MarkerSeek score 70.60

Primer available yes

Label ndhC-trnV-UAC

Feature Metadata

label_name: ndhC-trnV-UAC
feature_type: igs
region: LSC
length_bp: 1227
variable_sites: 64
pi: 0.0119
unique_haplotype_count: 40
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 70.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC-trnV-UAC_p1	AGATGCACTCCTATGAACGT	GAGAAGGTCTACGGTTCGAG	45.0	55.0	56.3	57.2	1264-1336 bp	1.0	68.1
ndhC-trnV-UAC_p2	AGATGCACTCCTATGAACGT	CGAGAAGGTCTACGGTTCGA	45.0	55.0	56.3	58.9	1265-1337 bp	1.0	67.4
ndhC-trnV-UAC_p3	GCAGAAACATAGATGCACTCC	GAGAAGGTCTACGGTTCGAG	47.6	55.0	57.0	57.2	1274-1346 bp	1.0	65.6
ndhC-trnV-UAC_p4	AGATGCACTCCTATGAACGT	AGAAGGTCTACGGTTCGAGC	45.0	55.0	56.3	59.2	1263-1335 bp	1.0	65.2
ndhC-trnV-UAC_p5	GCAGAAACATAGATGCACTCC	CGAGAAGGTCTACGGTTCGA	47.6	55.0	57.0	58.9	1275-1347 bp	1.0	64.9