Hotspot

ycf3-trnS-GGA

LSC · 44777-45708 · 932 bp

Back to Anaphalis

Pi 0.00742

MarkerSeek score 63.80

Primer available yes

Label ycf3-trnS-GGA

Feature Metadata

label_name: ycf3-trnS-GGA
feature_type: igs
region: LSC
length_bp: 932
variable_sites: 47
pi: 0.00742
unique_haplotype_count: 37
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 63.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf3-trnS-GGA_p1	TCTCCTGAGGTTGTCGGAAT	TTCCAATGCTACGCCTTCAA	50.0	45.0	58.1	57.8	1062-1076 bp	1.0	83.8
ycf3-trnS-GGA_p2	TCTCCTGAGGTTGTCGGAAT	GGATTCGAACCCTCGGTAAG	50.0	55.0	58.1	57.8	1100-1114 bp	1.0	83.4
ycf3-trnS-GGA_p3	TCTCCTGAGGTTGTCGGAAT	AGTTCCAATGCTACGCCTTC	50.0	50.0	58.1	58.3	1064-1078 bp	1.0	83.3
ycf3-trnS-GGA_p4	TCTCCTGAGGTTGTCGGAAT	CCTCGGTAAGCAAAAGCCTA	50.0	50.0	58.1	57.6	1090-1104 bp	1.0	82.0
ycf3-trnS-GGA_p5	TTCTCCTGAGGTTGTCGGAA	TTCCAATGCTACGCCTTCAA	50.0	45.0	58.3	57.8	1063-1077 bp	1.0	82.0