Hotspot

psbZ-trnG-UCC

LSC · 36185-36486 · 302 bp

Back to Anaphalis

Pi 0.00611

MarkerSeek score 36.90

Primer available yes

Label psbZ-trnG-UCC

Feature Metadata

label_name: psbZ-trnG-UCC
feature_type: igs
region: LSC
length_bp: 302
variable_sites: 14
pi: 0.00611
unique_haplotype_count: 11
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 36.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbZ-trnG-UCC_p1	TTGCTTCTCCTGATGGTTGG	ACTTCATTTTGGGCGGGTAG	50.0	50.0	57.8	58.2	494-572 bp	0.978723	78.4
psbZ-trnG-UCC_p2	TTGCTTCTCCTGATGGTTGG	GACTTCATTTTGGGCGGGTA	50.0	50.0	57.8	58.2	495-573 bp	0.978723	78.4
psbZ-trnG-UCC_p3	GCTTCTCCTGATGGTTGGTC	ACTTCATTTTGGGCGGGTAG	55.0	50.0	58.3	58.2	492-570 bp	0.978723	78.0
psbZ-trnG-UCC_p4	GCTTCTCCTGATGGTTGGTC	GACTTCATTTTGGGCGGGTA	55.0	50.0	58.3	58.2	493-571 bp	0.978723	78.0
psbZ-trnG-UCC_p5	TTGCTTCTCCTGATGGTTGG	ATGACTTCATTTTGGGCGGG	50.0	50.0	57.8	58.8	497-575 bp	0.978723	73.4