Hotspot

rpl14

Genome · 84205-84573 · 369 bp

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Pi 0.00271
MarkerSeek score 43.30
Primer available yes
Label rpl14

Feature Metadata

label_name
rpl14
feature_type
gene
region
Genome
length_bp
369
variable_sites
1
pi
0.00271
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
43.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl14_p1 ACGTGAGACACAATCTACTA TGCATTGAAATAACAGATTGAA 40.0 27.3 53.2 52.6 482 bp 1.0 40.2
rpl14_p2 ACGTGAGACACAATCTACTA TTGCATTGAAATAACAGATTGA 40.0 27.3 53.2 52.6 483 bp 1.0 40.2
rpl14_p3 TACGTGAGACACAATCTACT TGCATTGAAATAACAGATTGAA 40.0 27.3 53.2 52.6 483 bp 1.0 40.2
rpl14_p4 TACGTGAGACACAATCTACT TTGCATTGAAATAACAGATTGA 40.0 27.3 53.2 52.6 484 bp 1.0 40.2
rpl14_p5 ACGTGAGACACAATCTACTAA TGCATTGAAATAACAGATTGAA 38.1 27.3 54.1 52.6 482 bp 1.0 40.2