Hotspot

ccsA

Genome · 118679-119638 · 960 bp

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Pi 0.00208
MarkerSeek score 59.90
Primer available yes
Label ccsA

Feature Metadata

label_name
ccsA
feature_type
gene
region
Genome
length_bp
960
variable_sites
2
pi
0.00208
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
59.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ccsA_p1 GAGTGGCGGCATCTTCTAAA TCCTTGATTCAAACGGTTCTCA 50.0 40.9 58.0 57.9 1153 bp 1.0 63.3
ccsA_p2 GCTAAAGCATCTCGGTTCGA TCCTTGATTCAAACGGTTCTCA 50.0 40.9 58.1 57.9 1177 bp 1.0 62.9
ccsA_p3 GAGTGGCGGCATCTTCTAAA CCTTGATTCAAACGGTTCTCA 50.0 42.9 58.0 56.5 1152 bp 1.0 59.7
ccsA_p4 AGTGGCGGCATCTTCTAAAA TCCTTGATTCAAACGGTTCTCA 45.0 40.9 57.5 57.9 1152 bp 1.0 59.6
ccsA_p5 GCTAAAGCATCTCGGTTCGA CCTTGATTCAAACGGTTCTCA 50.0 42.9 58.1 56.5 1176 bp 1.0 59.2