Hotspot

rpl32-trnL

Genome · 117760-118523 · 764 bp

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Pi 0.00525
MarkerSeek score 62.00
Primer available yes
Label rpl32-trnL

Feature Metadata

label_name
rpl32-trnL
feature_type
igs
region
Genome
length_bp
764
variable_sites
4
pi
0.00525
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
62.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL_p1 CACCGGGCATTCAAAAAGTT AGCGTGTCTACCGATTTCAC 45.0 50.0 57.5 58.0 849-851 bp 1.0 76.3
rpl32-trnL_p2 CACCGGGCATTCAAAAAGTT CAGCGTGTCTACCGATTTCA 45.0 50.0 57.5 58.0 850-852 bp 1.0 76.3
rpl32-trnL_p3 CACCGGGCATTCAAAAAGTT TTTAGAAGATGCCGCCACTC 45.0 50.0 57.5 58.0 909-911 bp 1.0 76.1
rpl32-trnL_p4 TTCCACCGGGCATTCAAAAA AGCGTGTCTACCGATTTCAC 45.0 50.0 58.6 58.0 852-854 bp 1.0 75.8
rpl32-trnL_p5 TTTCCACCGGGCATTCAAAA AGCGTGTCTACCGATTTCAC 45.0 50.0 58.6 58.0 853-855 bp 1.0 75.8