Hotspot

clpP-clpP

Genome · 74488-75363 · 876 bp

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Pi 0
MarkerSeek score 61.00
Primer available yes
Label clpP-clpP

Feature Metadata

label_name
clpP-clpP
feature_type
igs
region
Genome
length_bp
876
variable_sites
0
pi
0
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.98
markerseek_score
61.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
clpP-clpP_p1 CTCGCTATCAACCTCTTGGC TACCTTTTCGGAGTCCTGGA 55.0 50.0 58.4 57.7 958-977 bp 1.0 75.8
clpP-clpP_p2 CTCGCTATCAACCTCTTGGC GTACCTTTTCGGAGTCCTGG 55.0 55.0 58.4 57.6 959-978 bp 1.0 75.2
clpP-clpP_p3 CTCGCTATCAACCTCTTGGC TAATGCCCATTGGTGTTCCA 55.0 45.0 58.4 57.4 982-1001 bp 1.0 73.3
clpP-clpP_p4 CTCGCTATCAACCTCTTGGC GAGAGGAAGATGCGGTTTGG 55.0 55.0 58.4 58.6 940-959 bp 1.0 73.3
clpP-clpP_p5 CTCGCTATCAACCTCTTGGC GGAGAGGAAGATGCGGTTTG 55.0 55.0 58.4 58.6 941-960 bp 1.0 73.3