Hotspot

rps12-clpP

Genome · 73136-73275 · 140 bp

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Pi 0.0143
MarkerSeek score 60.70
Primer available yes
Label rps12-clpP

Feature Metadata

label_name
rps12-clpP
feature_type
igs
region
Genome
length_bp
140
variable_sites
2
pi
0.0143
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
60.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps12-clpP_p1 CATCCTCTAAGAGCGGGAGA CCAAGCTCATGGCATTGTTG 55.0 50.0 58.0 58.0 284 bp 1.0 80.4
rps12-clpP_p2 CATCCTCTAAGAGCGGGAGA TGTTGATCTTGTAGCGGTCG 55.0 50.0 58.0 58.0 269 bp 1.0 80.4
rps12-clpP_p3 CATCCTCTAAGAGCGGGAGA TATGTCAGCAACAGAAGCCC 55.0 50.0 58.0 57.9 302 bp 1.0 79.5
rps12-clpP_p4 CATCCTCTAAGAGCGGGAGA GTTGATCTTGTAGCGGTCGA 55.0 50.0 58.0 57.7 268 bp 1.0 78.3
rps12-clpP_p5 ACATCCTCTAAGAGCGGGAG CCAAGCTCATGGCATTGTTG 55.0 50.0 58.3 58.0 285 bp 1.0 78.1