Hotspot

rbcL-accD

Genome · 59148-59888 · 741 bp

Back to Ananas

Pi 0.00271
MarkerSeek score 49.20
Primer available yes
Label rbcL-accD

Feature Metadata

label_name
rbcL-accD
feature_type
igs
region
Genome
length_bp
741
variable_sites
2
pi
0.00271
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
49.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rbcL-accD_p1 CTAGCAAATGGAGCCCTGAG CAACATCGAATTGAACCACCA 55.0 42.9 58.0 57.1 866-869 bp 1.0 64.9
rbcL-accD_p2 CTAGCAAATGGAGCCCTGAG ACAACATCGAATTGAACCACC 55.0 42.9 58.0 57.1 867-870 bp 1.0 64.9
rbcL-accD_p3 TGAAGCTAGCAAATGGAGCC CAACATCGAATTGAACCACCA 50.0 42.9 58.2 57.1 871-874 bp 1.0 63.2
rbcL-accD_p4 TGAAGCTAGCAAATGGAGCC ACAACATCGAATTGAACCACC 50.0 42.9 58.2 57.1 872-875 bp 1.0 63.2
rbcL-accD_p5 TCGACTTCGAACCGGTAGAT CAACATCGAATTGAACCACCA 50.0 42.9 58.3 57.1 809-812 bp 1.0 63.1