Hotspot

atpB-rbcL

Genome · 56910-57707 · 798 bp

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Pi 0.00251
MarkerSeek score 60.30
Primer available yes
Label atpB-rbcL

Feature Metadata

label_name
atpB-rbcL
feature_type
igs
region
Genome
length_bp
798
variable_sites
2
pi
0.00251
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
60.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
atpB-rbcL_p1 GGGGAAAAACGACATCCAGT AGTCTCTGTTTGTGGTGACA 50.0 45.0 58.1 56.6 927-928 bp 1.0 68.5
atpB-rbcL_p2 TGGGCCAATAATTTGAGCGA AGTCTCTGTTTGTGGTGACA 45.0 45.0 57.9 56.6 905-906 bp 1.0 68.2
atpB-rbcL_p3 TGAGCGATACACCCTAGGTT AGTCTCTGTTTGTGGTGACA 50.0 45.0 57.8 56.6 892-893 bp 1.0 68.1
atpB-rbcL_p4 TTGAGCGATACACCCTAGGT AGTCTCTGTTTGTGGTGACA 50.0 45.0 57.8 56.6 893-894 bp 1.0 68.1
atpB-rbcL_p5 CTGGGCCAATAATTTGAGCG AGTCTCTGTTTGTGGTGACA 50.0 45.0 57.5 56.6 906-907 bp 1.0 65.3