Hotspot

ndhC-trnV

Genome · 52914-53940 · 1027 bp

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Pi 0.00195
MarkerSeek score 59.90
Primer available yes
Label ndhC-trnV

Feature Metadata

label_name
ndhC-trnV
feature_type
igs
region
Genome
length_bp
1027
variable_sites
2
pi
0.00195
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
59.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhC-trnV_p1 CTAACCGGGGCTAAAACTCC AGAAGGTCTACGGTTCGAGT 55.0 50.0 58.0 57.8 1164-1165 bp 1.0 78.3
ndhC-trnV_p2 CTAACCGGGGCTAAAACTCC GAGCCCAATGCAACAAACAA 55.0 45.0 58.0 57.8 1251-1252 bp 1.0 78.0
ndhC-trnV_p3 CGGGGCTAAAACTCCTGAAA AGAAGGTCTACGGTTCGAGT 50.0 50.0 57.8 57.8 1159-1160 bp 1.0 77.0
ndhC-trnV_p4 CGGGGCTAAAACTCCTGAAA GAGCCCAATGCAACAAACAA 50.0 45.0 57.8 57.8 1246-1247 bp 1.0 76.7
ndhC-trnV_p5 TAACCGGGGCTAAAACTCCT AGAAGGTCTACGGTTCGAGT 50.0 50.0 58.3 57.8 1163-1164 bp 1.0 75.8