Hotspot

ycf3-trnS

Genome · 46684-47300 · 617 bp

Back to Ananas

Pi 0.00162
MarkerSeek score 59.70
Primer available yes
Label ycf3-trnS

Feature Metadata

label_name
ycf3-trnS
feature_type
igs
region
Genome
length_bp
617
variable_sites
1
pi
0.00162
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
59.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf3-trnS_p1 TTCTCCTGAAGTTGTCGGAA GTGGTCGATAACGTTGGTGA 45.0 50.0 56.4 57.9 831 bp 1.0 66.9
ycf3-trnS_p2 TTTCTCCTGAAGTTGTCGGA GTGGTCGATAACGTTGGTGA 45.0 50.0 56.4 57.9 832 bp 1.0 66.9
ycf3-trnS_p3 TTCTCCTGAAGTTGTCGGAA CCCTCGGTAAACAAAAGCCT 45.0 50.0 56.4 58.1 780 bp 1.0 66.7
ycf3-trnS_p4 TTTCTCCTGAAGTTGTCGGA CCCTCGGTAAACAAAAGCCT 45.0 50.0 56.4 58.1 781 bp 1.0 66.7
ycf3-trnS_p5 TTCTCCTGAAGTTGTCGGAA ACGGAAAGAGAGGGATTCGA 45.0 50.0 56.4 58.2 801 bp 1.0 66.2