Hotspot

psbC-trnS

Genome · 37605-37741 · 137 bp

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Pi 0.0511
MarkerSeek score 81.40
Primer available yes
Label psbC-trnS

Feature Metadata

label_name
psbC-trnS
feature_type
igs
region
Genome
length_bp
137
variable_sites
7
pi
0.0511
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
81.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbC-trnS_p1 GCTGCAGGGTTTGAAAAAGG GGTTGATAGCTCCGGTCTTG 50.0 55.0 58.1 58.1 283 bp 1.0 80.5
psbC-trnS_p2 CCTGTTCTTTCCATGACCCC GGTTGATAGCTCCGGTCTTG 55.0 55.0 58.2 58.1 244 bp 1.0 80.5
psbC-trnS_p3 GCTGCAGGGTTTGAAAAAGG ATTACCCCTGGGAGAGATGG 50.0 55.0 58.1 57.9 309 bp 1.0 79.8
psbC-trnS_p4 GCTGCAGGGTTTGAAAAAGG GATGGCCGAGTGGTTGATAG 50.0 55.0 58.1 58.1 294 bp 1.0 79.8
psbC-trnS_p5 CCTGTTCTTTCCATGACCCC ATTACCCCTGGGAGAGATGG 55.0 55.0 58.2 57.9 270 bp 1.0 79.8