Hotspot

ycf1

SSC · 122256-127337 · 5082 bp

Back to Anacyclus

Pi 0.00169

MarkerSeek score 38.90

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5082
variable_sites: 20
pi: 0.00169
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 38.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	GCATTTCCCCAAAAGTTGACA	TGTTTGAAAAACCTCTTTTGACT	42.9	30.4	57.5	55.4	204 bp	1.0	41.2
ycf1_p2	TGCTAGCATTTCCCCAAAAGT	TGTTTGAAAAACCTCTTTTGACT	42.9	30.4	58.1	55.4	209 bp	1.0	41.1
ycf1_p3	CCTTTTTGCTAGCATTTCCCC	TGTTTGAAAAACCTCTTTTGACT	47.6	30.4	57.7	55.4	215 bp	1.0	41.1
ycf1_p4	TGCTAGCATTTCCCCAAAAGT	TGTTTGAAAAACCTCTTTTGACTT	42.9	29.2	58.1	56.1	209 bp	1.0	41.1
ycf1_p5	TGCTAGCATTTCCCCAAAAGT	TTGTTTGAAAAACCTCTTTTGACT	42.9	29.2	58.1	56.1	210 bp	1.0	41.1