Hotspot

trnF-GAA-ndhJ

LSC · 47484-48181 · 698 bp

Back to Anacyclus

Pi 0.00301

MarkerSeek score 58.50

Primer available yes

Label trnF-GAA-ndhJ

Feature Metadata

label_name: trnF-GAA-ndhJ
feature_type: igs
region: LSC
length_bp: 698
variable_sites: 5
pi: 0.00301
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 58.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnF-GAA-ndhJ_p1	TGGGATAGCTCAGTCGGTAG	TGGATAGGATGGCCTTTACG	55.0	50.0	58.0	56.8	831-839 bp	1.0	71.4
trnF-GAA-ndhJ_p2	TGGGATAGCTCAGTCGGTAG	GGATAGGATGGCCTTTACGT	55.0	50.0	58.0	56.8	830-838 bp	1.0	71.4
trnF-GAA-ndhJ_p3	CCTCGTGTCACCAGTTCAAA	TGGATAGGATGGCCTTTACG	50.0	50.0	58.1	56.8	794-802 bp	1.0	71.2
trnF-GAA-ndhJ_p4	CCTCGTGTCACCAGTTCAAA	GGATAGGATGGCCTTTACGT	50.0	50.0	58.1	56.8	793-801 bp	1.0	71.2
trnF-GAA-ndhJ_p5	TGGGATAGCTCAGTCGGTAG	TGAAAGTTGGATAGGATGGCC	55.0	47.6	58.0	57.7	838-846 bp	1.0	70.8