Hotspot

atpI-atpH

LSC · 24957-26102 · 1146 bp

Back to Anacyclus

Pi 0.000349

MarkerSeek score 59.40

Primer available yes

Label atpI-atpH

Feature Metadata

label_name: atpI-atpH
feature_type: igs
region: LSC
length_bp: 1146
variable_sites: 1
pi: 0.000349
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpI-atpH_p1	TCCCTGTCATGTTCCTTGGA	ATAACGGAAGCGGCAGAAAT	50.0	45.0	58.3	58.0	1280-1285 bp	1.0	78.0
atpI-atpH_p2	TCCCTGTCATGTTCCTTGGA	TACCTTGACCAACTCCAGGT	50.0	50.0	58.3	57.9	1336-1341 bp	1.0	77.4
atpI-atpH_p3	TCCCTGTCATGTTCCTTGGA	AGCCAATCCAGCAGCAATAA	50.0	45.0	58.3	57.9	1296-1301 bp	1.0	77.1
atpI-atpH_p4	TCCCTGTCATGTTCCTTGGA	AATAACGGAAGCGGCAGAAA	50.0	45.0	58.3	58.2	1281-1286 bp	1.0	76.8
atpI-atpH_p5	TCCCTGTCATGTTCCTTGGA	GCAGTACCTTGACCAACTCC	50.0	55.0	58.3	58.2	1340-1345 bp	1.0	76.8