Hotspot

psbM-trnD-GUC

LSC · 10686-11348 · 663 bp

Back to Anacyclus

Pi 0.00242

MarkerSeek score 60.80

Primer available yes

Label psbM-trnD-GUC

Feature Metadata

label_name: psbM-trnD-GUC
feature_type: igs
region: LSC
length_bp: 663
variable_sites: 4
pi: 0.00242
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbM-trnD-GUC_p1	GGAACTAAAATGAACAGTGCAGT	CCTGTCAAGGCGGATGTTAC	39.1	55.0	57.6	58.6	761-764 bp	0.8	40.1
psbM-trnD-GUC_p2	AAAATGAACAGTGCAGTAGC	CCTGTCAAGGCGGATGTTAC	40.0	55.0	54.5	58.6	755-758 bp	0.8	39.6
psbM-trnD-GUC_p3	ACTAAAATGAACAGTGCAGTAGC	CCTGTCAAGGCGGATGTTAC	39.1	55.0	57.4	58.6	758-761 bp	0.8	38.7
psbM-trnD-GUC_p4	AGGAACTAAAATGAACAGTGCAG	CCTGTCAAGGCGGATGTTAC	39.1	55.0	57.3	58.6	762-765 bp	0.8	38.0
psbM-trnD-GUC_p5	GGAACTAAAATGAACAGTGCAGT	TTCAATAGGCTAGAGCACCG	39.1	50.0	57.6	57.1	782-785 bp	0.8	38.0