Hotspot

trnS-GCU-trnC-GCA

LSC · 8592-9294 · 703 bp

Back to Anacyclus

Pi 0.00341

MarkerSeek score 61.10

Primer available yes

Label trnS-GCU-trnC-GCA

Feature Metadata

label_name: trnS-GCU-trnC-GCA
feature_type: igs
region: LSC
length_bp: 703
variable_sites: 5
pi: 0.00341
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-trnC-GCA_p1	ACGGAAAGAGAGGGATTCGA	CCGGATTTGAACTGGGGAAA	50.0	50.0	58.2	58.1	854 bp	1.0	78.6
trnS-GCU-trnC-GCA_p2	ACGGAAAGAGAGGGATTCGA	AAAAGGATTTGCAGTCCCCC	50.0	50.0	58.2	58.4	836 bp	1.0	76.4
trnS-GCU-trnC-GCA_p3	CTTTCGTCCACTCAGCCATC	CCGGATTTGAACTGGGGAAA	55.0	50.0	58.6	58.1	790 bp	1.0	74.8
trnS-GCU-trnC-GCA_p4	AACGGAAAGAGAGGGATTCG	CCGGATTTGAACTGGGGAAA	50.0	50.0	57.3	58.1	855 bp	1.0	74.4
trnS-GCU-trnC-GCA_p5	CTTTCGTCCACTCAGCCATC	AAAAGGATTTGCAGTCCCCC	55.0	50.0	58.6	58.4	772 bp	1.0	72.6