Hotspot

trnK-UUU-rps16

LSC · 4347-5213 · 867 bp

Back to Anacyclus

Pi 0.00153
MarkerSeek score 61.60
Primer available yes
Label trnK-UUU-rps16

Feature Metadata

label_name
trnK-UUU-rps16
feature_type
igs
region
LSC
length_bp
867
variable_sites
3
pi
0.00153
unique_haplotype_count
5
primer_available
yes
alignment_reliability
0.98
markerseek_score
61.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-rps16_p1 CCGATGGTATGGACGAATCC TTGAAAAAGGTGCTCAGCCT 55.0 45.0 57.9 57.9 1012-1031 bp 1.0 80.5
trnK-UUU-rps16_p2 CGATGGTATGGACGAATCCC TTGAAAAAGGTGCTCAGCCT 55.0 45.0 57.9 57.9 1011-1030 bp 1.0 80.5
trnK-UUU-rps16_p3 AAAGCCGAGTACTCTACCGT TTGAAAAAGGTGCTCAGCCT 50.0 45.0 58.2 57.9 951-970 bp 1.0 79.9
trnK-UUU-rps16_p4 AAGCCGAGTACTCTACCGTT TTGAAAAAGGTGCTCAGCCT 50.0 45.0 58.2 57.9 950-969 bp 1.0 79.9
trnK-UUU-rps16_p5 CGATGGTATGGACGAATCCC AAAAGGTGCTCAGCCTACAG 55.0 50.0 57.9 57.8 1007-1026 bp 1.0 79.6