Hotspot

trnR-ACG-rrn5

IRa · 125815-126099 · 285 bp

Back to Anacolosa

Pi 0.122
MarkerSeek score 73.60
Primer available yes
Label trnR-ACG-rrn5

Feature Metadata

label_name
trnR-ACG-rrn5
feature_type
igs
region
IRa
length_bp
285
variable_sites
28
pi
0.122
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.80
markerseek_score
73.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnR-ACG-rrn5_p1 GTCCCGGTCCTGTTTCAAAT TGTCCCCTCCAGTCAAGAAT 50.0 50.0 58.1 58.0 704-927 bp 1.0 87.2
trnR-ACG-rrn5_p2 GTCCCGGTCCTGTTTCAAAT CCTTGTTCCTACATGACCCG 50.0 55.0 58.1 58.0 828-1055 bp 1.0 87.1
trnR-ACG-rrn5_p3 GTCCCGGTCCTGTTTCAAAT GCGGAAAAATAGCTCGATGC 50.0 50.0 58.1 58.0 526 bp 0.5 59.1
trnR-ACG-rrn5_p4 AAGGTAAAGTTGTGGGCGAG GCGGAAAAATAGCTCGATGC 50.0 50.0 58.1 58.0 397 bp 0.5 58.9
trnR-ACG-rrn5_p5 AAGGTAAAGTTGTGGGCGAG CCTTGTTCCTACATGACCCG 50.0 55.0 58.1 58.0 699 bp 0.5 58.9