Hotspot

ycf1-trnN-GUU

SSC · 125057-125356 · 300 bp

Back to Anacolosa

Pi 0.0621

MarkerSeek score 71.30

Primer available yes

Label ycf1-trnN-GUU

Feature Metadata

label_name: ycf1-trnN-GUU
feature_type: igs
region: SSC
length_bp: 300
variable_sites: 18
pi: 0.0621
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 71.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1-trnN-GUU_p1	AATGGGTGACGGTATTCTGC	ATTTGAAACAGGACCGGGAC	50.0	50.0	58.0	58.1	1256-1268 bp	1.0	83.1
ycf1-trnN-GUU_p2	TACCCTTGTCTCGGTTCCTT	ATTTGAAACAGGACCGGGAC	50.0	50.0	58.0	58.1	746 bp	0.5	59.1
ycf1-trnN-GUU_p3	TACCCTTGTCTCGGTTCCTT	CTCGCCCACAACTTTACCTT	50.0	50.0	58.0	58.1	875 bp	0.5	59.0
ycf1-trnN-GUU_p4	AATGGGTGACGGTATTCTGC	CTCGCCCACAACTTTACCTT	50.0	50.0	58.0	58.1	1397 bp	0.5	58.8
ycf1-trnN-GUU_p5	TACCCTTGTCTCGGTTCCTT	ACGACCAGTCAGTTAACAGC	50.0	50.0	58.0	57.8	532 bp	0.5	58.6