Hotspot

psaA-ycf3

LSC · 44507-45463 · 957 bp

Back to Anacolosa

Pi 0.0588

MarkerSeek score 78.10

Primer available yes

Label psaA-ycf3

Feature Metadata

label_name: psaA-ycf3
feature_type: igs
region: LSC
length_bp: 957
variable_sites: 50
pi: 0.0588
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.89
markerseek_score: 78.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaA-ycf3_p1	ACTTCTGGTTCCGGTGAA	ATCAAGCCGCTGAGTATTGG	50.0	50.0	55.6	58.0	990-1084 bp	1.0	52.4
psaA-ycf3_p2	ACTTCTGGTTCCGGTGAA	TTCGGAGGCTTGGTTCAATC	50.0	50.0	55.6	58.2	1007-1101 bp	1.0	51.3
psaA-ycf3_p3	ACTTCTGGTTCCGGTGAA	AGCCGCTGAGTATTGGAAAC	50.0	50.0	55.6	58.3	986-1080 bp	1.0	50.7
psaA-ycf3_p4	ACTTCTGGTTCCGGTGAA	CAAGCCGCTGAGTATTGGAA	50.0	50.0	55.6	58.3	988-1082 bp	1.0	50.7
psaA-ycf3_p5	ACTTCTGGTTCCGGTGAA	TTTCGGAGGCTTGGTTCAAT	50.0	45.0	55.6	57.7	1008-1102 bp	1.0	50.4