Hotspot

psbZ-trnG-GCC

LSC · 38728-39101 · 374 bp

Back to Anacolosa

Pi 0.0344

MarkerSeek score 69.80

Primer available yes

Label psbZ-trnG-GCC

Feature Metadata

label_name: psbZ-trnG-GCC
feature_type: igs
region: LSC
length_bp: 374
variable_sites: 11
pi: 0.0344
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.86
markerseek_score: 69.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbZ-trnG-GCC_p1	CTCCTGATGGTTGGTCAAGT	CGTCTTCTCCTTGGCAAAGA	50.0	50.0	57.1	57.8	471-525 bp	1.0	77.5
psbZ-trnG-GCC_p2	GGGTTAGTCTTTTTGGTGGGT	CGTCTTCTCCTTGGCAAAGA	47.6	50.0	58.0	57.8	412-466 bp	1.0	76.3
psbZ-trnG-GCC_p3	TGGGTTAGTCTTTTTGGTGGG	CGTCTTCTCCTTGGCAAAGA	47.6	50.0	58.0	57.8	413-467 bp	1.0	76.3
psbZ-trnG-GCC_p4	CTCCTGATGGTTGGTCAAGT	ACATTACTTCATCCTGGGCG	50.0	50.0	57.1	57.7	511-565 bp	1.0	76.0
psbZ-trnG-GCC_p5	CTCCTGATGGTTGGTCAAGT	CATTACTTCATCCTGGGCGG	50.0	55.0	57.1	58.4	510-564 bp	1.0	75.5