Hotspot

psbC-trnS-UGA

LSC · 37873-38089 · 217 bp

Back to Anacolosa

Pi 0.0507

MarkerSeek score 73.80

Primer available yes

Label psbC-trnS-UGA

Feature Metadata

label_name: psbC-trnS-UGA
feature_type: igs
region: LSC
length_bp: 217
variable_sites: 11
pi: 0.0507
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 73.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbC-trnS-UGA_p1	CCTGTTCTTTCCATGACCCC	GGTTGATAGCTCCGGTCTTG	55.0	55.0	58.2	58.1	324 bp	1.0	81.0
psbC-trnS-UGA_p2	CCTGTTCTTTCCATGACCCC	GATGGCCGAGTGGTTGATAG	55.0	55.0	58.2	58.1	335 bp	1.0	80.3
psbC-trnS-UGA_p3	CCTGTTCTTTCCATGACCCC	GGGTTCGAATCCCTCTCTCT	55.0	55.0	58.2	58.2	270 bp	1.0	80.2
psbC-trnS-UGA_p4	AGCTGCAGCAGGATTTGAAA	GGTTGATAGCTCCGGTCTTG	45.0	55.0	58.4	58.1	367 bp	1.0	78.9
psbC-trnS-UGA_p5	GCTGCAGCAGGATTTGAAAA	GGTTGATAGCTCCGGTCTTG	45.0	55.0	57.6	58.1	366 bp	1.0	78.4