Hotspot

rpl16

LSC · 81148-82507 · 1360 bp

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Pi 0.0137

MarkerSeek score 57.40

Primer available yes

Label rpl16

Feature Metadata

label_name: rpl16
feature_type: gene
region: LSC
length_bp: 1360
variable_sites: 38
pi: 0.0137
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 57.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl16_p1	ACACATCAATTCTCGAGCCC	AAATTGCAGGCCGTATCGAT	50.0	45.0	58.0	58.0	1823-1865 bp	1.0	84.0
rpl16_p2	ACACATCAATTCTCGAGCCC	CAGGCCGTATCGATGGAAAA	50.0	50.0	58.0	58.1	1817-1859 bp	1.0	83.8
rpl16_p3	ACACATCAATTCTCGAGCCC	ATCCAAATTGCAGGCCGTAT	50.0	45.0	58.0	57.9	1827-1869 bp	1.0	83.7
rpl16_p4	ACACATCAATTCTCGAGCCC	GTGCGTATACCGTTCGAACT	50.0	50.0	58.0	58.1	1724-1766 bp	1.0	83.7
rpl16_p5	ACACATCAATTCTCGAGCCC	GTCGAAGGGATTGCACGTAT	50.0	50.0	58.0	58.1	2142-2184 bp	1.0	83.2