Hotspot

ndhF-rpl32

SSC · 110176-111278 · 1103 bp

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Pi 0.0136

MarkerSeek score 62.70

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 1103
variable_sites: 36
pi: 0.0136
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 62.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	GGAAGGGTATGATCCATGCA	CGAATGCGCTTTTTGTAAATCG	50.0	40.9	57.0	57.9	1133-1190 bp	1.0	61.7
ndhF-rpl32_p2	GGAAGGGTATGATCCATGCA	TGCGCTTTTTGTAAATCGAAGT	50.0	36.4	57.0	57.8	1129-1186 bp	1.0	61.1
ndhF-rpl32_p3	TGGAAGGAAGGGTATGATCCA	CGAATGCGCTTTTTGTAAATCG	47.6	40.9	57.9	57.9	1138-1195 bp	1.0	60.2
ndhF-rpl32_p4	TGGAAGGAAGGGTATGATCCA	TGCGCTTTTTGTAAATCGAAGT	47.6	36.4	57.9	57.8	1134-1191 bp	1.0	59.6
ndhF-rpl32_p5	AGGAAGGGTATGATCCATGC	CGAATGCGCTTTTTGTAAATCG	50.0	40.9	56.7	57.9	1134-1191 bp	1.0	59.3