Hotspot

psaJ-rpl33

LSC · 67221-67469 · 249 bp

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Pi 0.0254
MarkerSeek score 62.70
Primer available yes
Label psaJ-rpl33

Feature Metadata

label_name
psaJ-rpl33
feature_type
igs
region
LSC
length_bp
249
variable_sites
16
pi
0.0254
unique_haplotype_count
6
primer_available
yes
alignment_reliability
0.98
markerseek_score
62.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psaJ-rpl33_p1 TTTTCCCAGATGCGTTGACA TCGCAATTCCAACCGACTAG 45.0 50.0 58.0 58.0 427-439 bp 0.833333 80.4
psaJ-rpl33_p2 ATGCGTTGACATTCCCCTTT TCGCAATTCCAACCGACTAG 45.0 50.0 58.1 58.0 418-430 bp 0.833333 80.1
psaJ-rpl33_p3 AGTTCAGTAGGAAGGGGCAT TCGCAATTCCAACCGACTAG 50.0 50.0 58.0 58.0 741-768 bp 0.833333 79.9
psaJ-rpl33_p4 GGAATGCCCAGTTCAGTAGG TCGCAATTCCAACCGACTAG 55.0 50.0 58.0 58.0 750-777 bp 0.833333 79.8
psaJ-rpl33_p5 GTTCAGTAGGAAGGGGCATG TCGCAATTCCAACCGACTAG 55.0 50.0 58.0 58.0 740-767 bp 0.833333 79.8