Hotspot

petA-psbJ

LSC · 62809-63751 · 943 bp

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Pi 0.0164

MarkerSeek score 62.90

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 943
variable_sites: 39
pi: 0.0164
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 62.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	CGAGAAGGTTCAACTGTCCG	GGATTAGGCTCATCGCTGTAG	55.0	52.4	58.6	58.0	973-1002 bp	1.0	73.4
petA-psbJ_p2	CGAGAAGGTTCAACTGTCCG	GGATTAGGCTCATCGCTGTA	55.0	50.0	58.6	56.9	973-1002 bp	1.0	72.9
petA-psbJ_p3	GAGAAGGTTCAACTGTCCGA	GGATTAGGCTCATCGCTGTAG	50.0	52.4	57.2	58.0	972-1001 bp	1.0	71.6
petA-psbJ_p4	TCGAGAAGGTTCAACTGTCC	GGATTAGGCTCATCGCTGTAG	50.0	52.4	57.2	58.0	974-1003 bp	1.0	71.6
petA-psbJ_p5	GAGAAGGTTCAACTGTCCGA	GGATTAGGCTCATCGCTGTA	50.0	50.0	57.2	56.9	972-1001 bp	1.0	71.1